Review



mouse monoclonal antibody against glut4 1f8  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    R&D Systems mouse monoclonal antibody against glut4 1f8
    Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The <t>GLUT4</t> dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.
    Mouse Monoclonal Antibody Against Glut4 1f8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody against glut4 1f8/product/R&D Systems
    Average 90 stars, based on 33 article reviews
    mouse monoclonal antibody against glut4 1f8 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "A novel phenolic formulation for treating hepatic and peripheral insulin resistance by regulating GLUT4-mediated glucose uptake"

    Article Title: A novel phenolic formulation for treating hepatic and peripheral insulin resistance by regulating GLUT4-mediated glucose uptake

    Journal: Journal of Traditional and Complementary Medicine

    doi: 10.1016/j.jtcme.2021.08.004

    Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The GLUT4 dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.
    Figure Legend Snippet: Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The GLUT4 dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.

    Techniques Used: Incubation

    Effect of phenolic combination and anti-diabetic drugs on the translocation of GLUT4 to plasma membrane and the related intracellular signaling molecules including phosphorylated AKT ( p -AKT) and ACC ( p -ACC) in insulin-resistant L6 myotubes. Palmitate-treated myotubes were incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. The cell lysates were used for preparation of a plasma membrane fraction as described under “Methods”. Protein (50 μg) was resolved by SDS-PAGE and Western blotted for the proteins shown. Detection was by enhanced chemiluminescence, and a representative blot is depicted. Anti-β-actin was used as protein loading control of whole homogenate.
    Figure Legend Snippet: Effect of phenolic combination and anti-diabetic drugs on the translocation of GLUT4 to plasma membrane and the related intracellular signaling molecules including phosphorylated AKT ( p -AKT) and ACC ( p -ACC) in insulin-resistant L6 myotubes. Palmitate-treated myotubes were incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. The cell lysates were used for preparation of a plasma membrane fraction as described under “Methods”. Protein (50 μg) was resolved by SDS-PAGE and Western blotted for the proteins shown. Detection was by enhanced chemiluminescence, and a representative blot is depicted. Anti-β-actin was used as protein loading control of whole homogenate.

    Techniques Used: Translocation Assay, Membrane, Incubation, SDS Page, Western Blot



    Similar Products

    mouse monoclonal antibody against glut4 1f8  (R&D Systems)
    90
    R&D Systems mouse monoclonal antibody against glut4 1f8
    Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The <t>GLUT4</t> dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.
    Mouse Monoclonal Antibody Against Glut4 1f8, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody against glut4 1f8/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    mouse monoclonal antibody against glut4 1f8 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The GLUT4 dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: A novel phenolic formulation for treating hepatic and peripheral insulin resistance by regulating GLUT4-mediated glucose uptake

    doi: 10.1016/j.jtcme.2021.08.004

    Figure Lengend Snippet: Effect of phenolic combination and anti-diabetic drugs on IDV-pretreated insulin-resistant L6 myotubes. Palmitate-treated L6 myotubes were stimulated for 5 min without or with 100 μM IDV, then incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. Data present the 2DG uptake influenced by palmitate pretreatment only (white bars) and palmitate along with IDV treatment (closed bars). Results are the means +SD of five independent experiments. Different superscript letters indicate significant differences at p < 0.05. The GLUT4 dependent effect above basal was calculated for each stimulator. # p < 0.05 compared with GLUT4 effect in cells not treated with IDV.

    Article Snippet: Mouse monoclonal antibody against GLUT4 (clone 1F8) was purchased from R&D Systems (Minneapolis, MN, USA).

    Techniques: Incubation

    Effect of phenolic combination and anti-diabetic drugs on the translocation of GLUT4 to plasma membrane and the related intracellular signaling molecules including phosphorylated AKT ( p -AKT) and ACC ( p -ACC) in insulin-resistant L6 myotubes. Palmitate-treated myotubes were incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. The cell lysates were used for preparation of a plasma membrane fraction as described under “Methods”. Protein (50 μg) was resolved by SDS-PAGE and Western blotted for the proteins shown. Detection was by enhanced chemiluminescence, and a representative blot is depicted. Anti-β-actin was used as protein loading control of whole homogenate.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: A novel phenolic formulation for treating hepatic and peripheral insulin resistance by regulating GLUT4-mediated glucose uptake

    doi: 10.1016/j.jtcme.2021.08.004

    Figure Lengend Snippet: Effect of phenolic combination and anti-diabetic drugs on the translocation of GLUT4 to plasma membrane and the related intracellular signaling molecules including phosphorylated AKT ( p -AKT) and ACC ( p -ACC) in insulin-resistant L6 myotubes. Palmitate-treated myotubes were incubated with DMSO (0.1 %), phenolic combination (5 μM EGEG + 10 μM RSV + 25 μM FER), metformin (1 mM) or AICAR (2 mM) for 15 min. The cell lysates were used for preparation of a plasma membrane fraction as described under “Methods”. Protein (50 μg) was resolved by SDS-PAGE and Western blotted for the proteins shown. Detection was by enhanced chemiluminescence, and a representative blot is depicted. Anti-β-actin was used as protein loading control of whole homogenate.

    Article Snippet: Mouse monoclonal antibody against GLUT4 (clone 1F8) was purchased from R&D Systems (Minneapolis, MN, USA).

    Techniques: Translocation Assay, Membrane, Incubation, SDS Page, Western Blot